Biotechnology: Patch-clamp method gets a makeover
نویسندگان
چکیده
منابع مشابه
A method of patch clamp recording in hyperbaric oxygen.
A method of patch clamp recording in hyperbaric oxygen is described which is derived from a method for patch clamping at high hydrostatic pressure. Excised patches equilibrate with hyperbaric oxygen within 10 minutes. Experiments with BK channels expressed in HEK 293 cells showed that 1 MPa O2 caused an increase in the ionic permeability of the patch, an effect accelerated in the presence of fe...
متن کاملAutomated planar patch-clamp.
Ion channels are integral membrane proteins that regulate the flow of ions across the plasma membrane and the membranes of intracellular organelles of both excitable and non-excitable cells. Ion channels are vital to a wide variety of biological processes and are prominent components of the nervous system and cardiovascular system, as well as controlling many metabolic functions. Furthermore, i...
متن کاملPatch-clamp recording on a chip
Cristian Ionescu-Zanetti et al. have developed a technique for electrophysiological recording of whole mammalian cells on a microf luidic chip. The technique permits multiplexed recording of ion channel voltages, while leaving the cells visible for microscopic inspection and manipulation. The device records through many junctions along the microf luidic channel while a microscope observes from ...
متن کاملWhole-Cell Patch-Clamp Recordings
The patch-clamp recording technique, which measures ionic currents under voltage clamp, was designed to study small patches of membranes in which near perfect control of the transmembrane voltage can be achieved readily. The recent application of patch-clamp methodology to the analysis of whole-cell current actually defies many of the original design requirements. Nevertheless, whole-cell recor...
متن کاملPatch clamp detection in capillary electrophoresis.
We describe a capillary electrophoresis-patch clamp (CE-PC) analysis of biomolecules that activate ligand-gated ion channels. CE-PC offers a powerful means for identifying receptor ligands based on the combination of the characteristic receptor responses they evoke and their differential electrophoretic migration rates. Corner frequencies, membrane reversal potentials, and mean and unitary sing...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2002
ISSN: 0003-2700,1520-6882
DOI: 10.1021/ac0221029